Analysis Note

ControlHuman stomach, thymus, and uterus tissue lysates

Application

Immunohistochemistry Analysis: 2 µg/mL from a representative lot detected TRIM5 alpha in human stomach tissue.

Immunofluorescence Analysis: 10 µg/mL from a representative lot detected TRIM5 alpha in human stomach cells.

Detect Tripartite motif-containing protein 5 using this rabbit polyclonal antibody, Anti-TRIM5 alpha Antibody validated for use in western blotting, IHC & Immunofluorescence.

General description

Tripartite motif-containing protein 5 (TRIM5), also known as RING finger protein 88 (RNF88), is a member of the TRIM/RBCC family defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. TRIM5 isoform alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection.

Immunogen

KLH-conjugated linear peptide corresponding to the intermediate domain of human TRIM5 alpha.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blotting in human stomach, thymus, and uterus tissue lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected TRIM5 alpha in 15 µg of human stomach, thymus, and uterus tissue lysates.

Specificity

This antibody recognizes the intermediate domain of TRIM5 alpha.

Target description

~55 kDa observed. Uniprot describes 6 isoforms ranging between ~30 kDa and ~56 kDa Uncharacterized bands may appear in some lysates.